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Imaging & Photomanipulation - Resources

Publications - last updated December, 2008

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Brown CM, Dalal RB, Hebert B, Digman MA, Horwitz AR, Gratton E. Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. J Microsc 2008; 229 (Pt 1):78-91 PubMed | CMC Update article.
  • Dalal RB, Digman MA, Horwitz AF, Vetri V, Gratton E. Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc Res Tech 2007; 71 (1):69-81. PubMed | CMC Update article.
  • Digman MA, Dalal R, Horwitz AF, Gratton E. Mapping the number of molecules and brightness in the laser scanning microscope. Biophys J 2008; 94 (6):2320-32 PubMed | CMC Update article.
  • Digman M, Caiolfa VR, Zamai M, Gratton E. The Phasor approach to fluorescence lifetime imaging analysis. Biophys J 2008; 94 (2):L14-6 PubMed | CMC Update article.
  • Digman MA, Brown CM, Horwitz AF, Mantulin WW, Gratton E. Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophys J 2008; 94 (7):2819-31 PubMed | CMC Update article.
  • Jacobson K , Rajfur Z, Vitriol E, Hahn K. Chromophore-assisted laser inactivation in cell biology. Trends Cell Biol. 2008 Sep;18(9):443-50. PubMed | CMC Update article
  • Murase S, Cho C, White JM, Horwitz AF. ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb. Eur J Neurosci 2008; 27 (7):1585-95 PubMed | CMC Update article.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Aemissegger A, Carrigan C, Imperiali B. Caged O-phosphorothioyl amino acids as building blocks for Fmoc-based solid phase peptide synthesis. Tetrahedron 2007; (63):6185-90
  • Loitto VM, Huang C, Sigal YJ, Jacobson K. Filopodia are induced by aquaporin-9 expression. Exp Cell Res 2007; 313 (7):1295-306 PubMed | CMC Update article.
  • Schober JM, Komarova YA, Chaga OY, Akhmanova A, Borisy GG. Microtubule-targeting-dependent reorganization of filopodia. J Cell Sci 2007; 120 (Pt 7):1235-44 PubMed | CMC Update article.
  • Sidani M, Wessels D, Mouneimne G, Ghosh M, Goswami S, Sarmiento C, Wang W, Kuhl S, El-Sibai M, Backer JM, Eddy R, Soll D, Condeelis J. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells. J Cell Biol 2007; 179 (4):777-91 PubMed | CMC Update article.
  • van Rheenen J, Song X, van Roosmalen W, Cammer M, Chen X, Desmarais V, Yip SC, Backer JM, Eddy RJ, Condeelis JS. EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells. J Cell Biol 2007; 179 (6):1247-59 PubMed | CMC Update article.
  • Vitriol EA, Uetrecht AC, Shen F, Jacobson K, Bear JE. Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins. Proc Natl Acad Sci U S A 2007; 104(16):6702-7. PubMed | CMC Update article.
  • Weinreb G, Cao Y. Fuzzy Causal Mapping (F-CMAP) - A Proposal to Develop a New Systems Biology Tool. Information Sci. e-Pub 2007; 144-156. DOI 10.1142/9789812709677_0022

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW. Probing the integrin-actin linkage using high-resolution protein velocity mapping. J Cell Sci 2006; 119 (Pt 24):5204-14 PubMed | CMC Update article.
  • Lagerholm BC, Averett L, Weinreb GE, Jacobson K, Thompson NL. Analysis Method for Measuring Submicroscopic Distances with Blinking Quantum Dots. Biophys J 2006; 91 (8):3050-60 PubMed | CMC Update article.
  • Weinreb G, Elston T, Jacobson K. The causal map as a tool to mechanistically interpret phenomena in cell motility: application to cortical oscillations in spreading cells. Cell Motil Cytoskeleton. 2006; 63 (9):523-32 PubMed | CMC Update article.
  • Wang YL, Hahn KM, Murphy RF, Horwitz AF. From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006. J Cell Biol 2006; 174 (4):481-4 PubMed | CMC Update article.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Carbonell WS, Murase S, Horwitz AF, Mandell JW. Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study. J Neurosci. 2005; 25(30):7040-7. PubMed
  • Carbonell WS, Murase SI, Horwitz AF, Mandell JW. Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J Neuroinflammation. 2005; 2(1):5. PubMed
  • Damljanovic V. Lagerholm BC, Jacobson K. Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. BioTech. 2005; 39(6):847-51. PubMed
  • Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E. Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys J. 2005; 89(2):1317-27. PubMed | CMC Update
  • Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E. Fluctuation correlation spectroscopy with a laser-scanning microscope: exploring the hidden time structure. Biophys J. 2005; 88(5):L33-L36. PubMed | CMC Update article.
  • Hebert B, Costantino S, Wiseman PW. Spatiotemporal Image Correlation Spectroscopy (STICS) Theory, Verification, and Application to Protein Velocity Mapping in Living CHO Cells. Biophys J. 2005; 88(5):3601-3614. PubMed
  • Humphrey D, Rajfur Z, Vazquez ME, Scheswohl D, Schaller MD, Jacobson K, Imperiali B. In situ photoactivation of a caged phosphotyrosine peptide derived from FAK temporarily halts lamellar extension of single migrating tumor cells. J Biol Chem. 2005; 280(23):22091-101. PubMed
  • Kandere-Grzybowska K, Campbell C, Komarova Y, Grzybowski BA, Borisy GG. Molecular dynamics imaging in micropatterned living cells. Nat Methods. 2005; 2(10):739-41. PubMed
  • Rothman DM, Petersson EJ, Vazquez ME, Brandt GS, Dougherty DA, Imperiali B. Caged phosphoproteins. J Am Chem Soc. 2005; 127(3):846-7. PubMed

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Huang C, Borchers CH, Schaller MD, Jacobson K. Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells. J Cell Biol 2004; 164 (4):593-602 PubMed
  • Huang C, Jacobson K, Schaller MD. MAP kinases and cell migration. J Cell Sci 2004; 117 (Pt 20):4619-28 PubMed
  • Murase S, Horwitz AF.Directions in cell migration along the rostral migratory stream: the pathway for migration in the brain.Curr Top Dev Biol. 2004; 61:135-52. PubMed
  • Nguyen A, Rothman DM, Stehn J, Imperiali B, Yaffe MB. Caged Phosphopeptides Reveal a Temporal Role for 14-3-3 in G1 Arrest and S-Phase Checkpoint Function. Nat Biotechnol. 2004; 22(8):993-1000. PubMed
  • Pomorski P, Watson JM, Haskill S, Jacobson KA. How adhesion, migration, and cytoplasmic calcium transients influence interleukin-1beta mRNA stabilization in human monocytes. Cell Motil Cytoskeleton 2004; 57 (3):143-57 PubMed.
  • Roy P, Jacobson K. Overexpression of profilin reduces the migration of invasive breast cancer cells. Cell Motil Cytoskeleton 2004; 57 (2):84-95 PubMed.
  • Wiseman PW, Brown CM, Webb DJ, Hebert B, Johnson NL, Squier JA, Ellisman MH, Horwitz AF. Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy. J Cell Sci. 2004; 117(Pt 23):5521-5534. PubMed

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Rothman DM, Vazquez ME, Vogel EM, Imperiali B. Caged phospho-amino acid building blocks for solid-phase peptide synthesis. J Org Chem. 2003; 68(17):6795-8. PubMed
  • Vazquez ME, Nitz M, Stehn J, Yaffe MB, Imperiali B. Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations. J Am Chem Soc. 2003; 125(34):10150-1. PubMed
  • Webb DJ, Brown CM, Horwitz AF. Illuminating adhesion complexes in migrating cells: moving toward a bright future. Curr Opin Cell Biol. 2003; 15(5):614-20. PubMed

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Mandell JW, Manabe R, Horwitz AF, Baumgart JP. Fluorescence imaging of mobility shifts: an expression cloning method for identification of cell signaling targets. Lab Invest 2002; 82 (12):1631-6 PubMed
  • Rajfur Z, Roy P, Otey C, Romer L, Jacobson K. Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins. Nat Cell Biol. 2002; 4(4):286-93. PubMed
  • Rothman DM, Vazquez ME, Vogel EM, Imperiali B. General method for the synthesis of caged phosphopeptides: tools for the exploration of signal transduction pathways. Org Lett. 2002; 4(17):2865-8. PubMed
  • Roy P, Rajfur Z, Pomorski P, Jacobson K. Microscope-based techniques to study cell adhesion and migration. Nat Cell Biol. 2002; 4(4):E91-6. PubMed
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Protocols/Software

Patterned Substrates for Imaging Living Cells

    The Borisy laboratory has developed novel micropatterning approaches that overcome many of the limitations associated with micropatterned substrates based on inert gold layers. Using an anisotropic solid microetching (ASOMIC) procedure, molecular dynamics imaging by wide-field and total internal reflection fluorescence (TIRF) microscopy of living mammalian cells and correlative platinum replica electron microscopy can be achieved. The protocols describing the production of these micropatterned substrates are described in detail in their October 2005 publication in Nature Methods. (Kandere-Grzybowska et al., 2005. Molecular dynamics imaging in micropatterned living cells. Nat Methods. 2005 Oct;2(10):739-41. PubMed)

Image Correlation Microscopy

Traction Assay Protocols (see also Damljanovic et al., 2005)

CALI Protocol (see also Rajfur et al., 2002)

Caged Phosphopeptide & Phosphoprotein Chemistry

  • Rothman et al., 2002 - Details of the solid-phase synthesis of cpErk, cpChk2 and cpPax, quantum yield determinations and 31P MAS NMR experiments are availablethrough the American Chemical Society* using journal reference OL0262587.
  • Rothman et al., 2003 - Details on 1H NMP and 13C NMR spectra for all compounds and general experimental information are availablethrough the American Chemical Society* using journal reference JO0344891.
  • Nguyen et al., 2004 - Supporting information for this paper is available by visiting Nature Biotechnology.

Environment - Sensitive Fluorophore Chemistry

  • Vazquez et al., 2003 - Details of the characterization of peptides 2,3 and 4 fluorescence spectra, HLPC traces and molecular modeling are available through the American Chemical Society* using journal reference JA0351847.

* Electronic supporting information files for articles appearing in ACS journals are available free of charge and without subscription via the Internet at http://pubs.acs.org. All files are copyrighted by the American Chemical Society but may be downloaded for personal use.

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