Cell Migration Gateway Logo

Discovery - Activities

Objectives

  • to use genetic and expression strategies in genetically tractable organisms to identify migration related genes
  • to use expression cloning strategies to identify proteins involved in cell migration
  • to screen chemical libraries for reagents that perturb migration, adhesion or cytoskeletal organization

Collaboration and Release Policy

Genetic Approaches | Gene Screens | Chemical Screens

Genetic Approaches

Organism Approach Status / Publication Contact (Collaborators)
D. melanogaster identification of genes upregulated in migrating cells Completed2736 examined, genes predicted to control actin dynamics and endocytic and secretory pathways are overrepresented in the migratory cell transcriptome
Completed - Data available		 Montell
D. melanogaster identification of genes encoding cytoskeleton-associated proteins enriched in migratory cells Completed45 genes enriched in migratory cells
Completed - Data available		 Montell
D. melanogaster identification of migratory cell enriched genes encoding proteins associated with vesicle traffic Completed47 genes enriched in migratory cells, encoding vesicle associated proteins
Completed - Data available		 Montell
C. elegans RNAi screen for DTC migration defects CompletedAnalysis of 16,758 RNA-interference depletion experiments identified 99 genes required for distal tip cell migration
Completed - Data available		 Schwarzbauer
D. melanogaster Collection of published cell migration genes in Drosophila (multiple cell types) CompletedRac, Shg and Stat92e are implicated in both primordial germ cell and in border cell migration
Completed - Primordial germ cell genes, Border cell genes		 Montell
D. melanogaster identification of downstream targets of migration-related transcription factors Identified 149 genes expressed at a lower level in border cells isolated from slbo mutants
Data available		 Montell
D. melanogaster matching border cell genes with migration defective mutants In progress Montell
D. melanogaster identification of genes that alter S2 morphology using large scale RNAi screening and high resolution/high-throughput microscopy In progress Vale
D. melanogaster characterization of the proteins identified in the high throughput screen In progress Vale
C. elegans Determine cell autonomy of 99 DTC migration genes Proposed Schwarzbauer
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Gene Screens

Phenotype Screen Target Cell Library (cDNA/siRNA) Status / Publication Contact (Collaborators)
Migration Transwell assay for induction of migration MCF-10A expressing activated ErbB2 BC-1000 cDNA 39 out of 41 genes increase migratory behavior in MCF-10A expressing activated ErbB2
Completed - Data available		 Brugge (LaBaer)
Migration Transwell assay for induction of migration MCF-10A MCF-7 cDNA 6 genes identified that increase migratory behavior
Completed - Data available		 Brugge
Migration Transwell assay for induction of migration MCF-10A expressing CSF-1R MCF-7 cDNA 4 genes identified that increase migratory behavior in MCF-10A expressing CSF-1R
Completed - Data available		 Brugge
Migration Transwell assay for induction of migration MCF-10A expressing CSF-1R and stimulated with CSF MCF-7 cDNA 4 genes identified that increase migratory behavior in MCF-10A expressing CSF-1R and stimulated with CSF
Completed - Data available		 Brugge
Migration Transwell assay for induction of migration MCF-10A expressing activated ErbB2 MCF-7 cDNA 3 genes identified that increase migratory behavior in MCF-10A expressing activated ErbB2
Completed - Data available		 Brugge
Migration Phagokinetic track assay (PKT) MCF-7 BC-1000 cDNA Data available after paper is accepted
Completed - Data available		 Geiger (S.Naffar Abu-Amara), Brugge
Structure Localization REF52 GFP-tagged HT1080 cDNA clones identified that localize to migration related structures
Completed - Data available - Manabe et al., 2002		 Horwitz
Structure Cytoskeletal localization HeLa with paxillin-YFP reporter siRNA-kinases Effect gene knockdown of 3 kinases on the morphology of focal adhesions is shown
Completed - Data available		 Geiger (S. Winograd-Katz), Brugge
Structure Morphological perturbations HeLa with paxillin-YFP reporter siRNA-phosphatases Effect of gene knockdown of 168 phosphatases on the morphology of focal adhesions is shown
Completed - Data available		 Geiger (S. Winograd-Katz), Brugge
Structure Morphological perturbations HeLa with paxillin-YFP reporter siRNA-FA proteins Effect of knockdown of 296 Migration-related genes on the morphology of focal adhesion is shown
Completed - Data available		 Geiger, Brugge
Migration Phagokinetic track assay (PKT) MCF-7 MDA-MB-231 cDNA Paper submitted for publication, data available upon request
Completed - Data available		 Geiger (S.Naffar Abu-Amara), Brugge
Structure Cytoskeletal localization REF52 YFP-tagged rat cDNA The localization to migratory structures has been identified for 202 clones
Completed - Data available		 Geiger (I. Lavelin)
Invasive Cell translocation in response to metastasis signal PyMT and MTLn3   Genes identified characteristic of the "invasion signature"
Completed - Data available		 Condeelis
Migration Transwell assay for induction of migration in the absence of a functional PI3K pathway MCF-10A stimulated with EGF + PI3K inhibition with LY294002 MDA-MB-231 cDNA Completed - no hits Brugge
Migration Phagokinetic track assay (PKT-B) MCF-10A siRNA-kinases, phosphatases and custom set Effects of siRNA-induced gene knockdown include decrease in focal adhesion number and change in distribution throughout the cell
Completed - Data available		 Geiger
Migration Wound healing assay MCF-10A siRNA-kinases, phosphatases and custom set 66 genes identified that positively or negatively regulate cell migration
Completed - Data available Simpson et al, 2008 		Brugge
Structure Cytoskeletal localization REF52 YFP-tagged arabidopsis cDNA Nearing completion Sadot & Kam

Methods for validation of siRNA targeting sequences - Gary Borisy.

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Chemical Screens

Phenotype Screen Target Cell Library Status/Publication Lead laboratory (Collaborators)
Structure Morphological perturbations REF52 with YFP paxillin Tel Aviv HTS Natural Products 17 compounds identified that perturb migration-related structures
Completed - Data available		 Geiger (Y. paran)
Structure Morphological perturbations REF52 with YFP paxillin Chem Bridge & Chemical Diversity 102 compounds identified that perturb migration-related structures
Completed - Data available		 Geiger (Y. paran)
Structure Phagokinetic track assay (PKT) MCF-7 and MDA-231-MB Tel Aviv HTS Natural Products Initialized Geiger (S. Naffar Abu-Amara)
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